diff --git a/.changes/add-cli-matches-from.md b/.changes/add-cli-matches-from.md
new file mode 100644
index 00000000..4703a5ba
--- /dev/null
+++ b/.changes/add-cli-matches-from.md
@@ -0,0 +1,6 @@
+---
+"cli": minor
+"cli-js": minor
+---
+
+Added `Cli.matches_from(args)`. This can be combined with the `args` passed to the callback of `tauri_plugin_single_instance::init` to parse the command line arguments passed to subsequent instances of the application.
diff --git a/plugins/cli/src/lib.rs b/plugins/cli/src/lib.rs
index 3e144376..e927a348 100644
--- a/plugins/cli/src/lib.rs
+++ b/plugins/cli/src/lib.rs
@@ -29,7 +29,11 @@ pub struct Cli<R: Runtime>(PluginApi<R, Config>);
 
 impl<R: Runtime> Cli<R> {
     pub fn matches(&self) -> Result<parser::Matches> {
-        parser::get_matches(self.0.config(), self.0.app().package_info())
+        parser::get_matches(self.0.config(), self.0.app().package_info(), None)
+    }
+
+    pub fn matches_from(&self, args: Vec<String>) -> Result<parser::Matches> {
+        parser::get_matches(self.0.config(), self.0.app().package_info(), Some(args))
     }
 }
 
diff --git a/plugins/cli/src/parser.rs b/plugins/cli/src/parser.rs
index 64ec2fb7..466885f1 100644
--- a/plugins/cli/src/parser.rs
+++ b/plugins/cli/src/parser.rs
@@ -19,7 +19,7 @@ use std::collections::HashMap;
 mod macros;
 
 /// The resolution of a argument match.
-#[derive(Default, Debug, Serialize)]
+#[derive(Default, Debug, Serialize, Clone)]
 #[non_exhaustive]
 pub struct ArgData {
     /// - [`Value::Bool`] if it's a flag,
@@ -33,7 +33,7 @@ pub struct ArgData {
 }
 
 /// The matched subcommand.
-#[derive(Default, Debug, Serialize)]
+#[derive(Default, Debug, Serialize, Clone)]
 #[non_exhaustive]
 pub struct SubcommandMatches {
     /// The subcommand name.
@@ -43,7 +43,7 @@ pub struct SubcommandMatches {
 }
 
 /// The argument matches of a command.
-#[derive(Default, Debug, Serialize)]
+#[derive(Default, Debug, Serialize, Clone)]
 #[non_exhaustive]
 pub struct Matches {
     /// Data structure mapping each found arg with its resolution.
@@ -79,7 +79,11 @@ impl Matches {
 ///     Ok(())
 ///   });
 /// ```
-pub fn get_matches(cli: &Config, package_info: &PackageInfo) -> crate::Result<Matches> {
+pub fn get_matches(
+    cli: &Config,
+    package_info: &PackageInfo,
+    args: Option<Vec<String>>,
+) -> crate::Result<Matches> {
     let about = cli
         .description()
         .unwrap_or(&package_info.description.to_string())
@@ -92,7 +96,14 @@ pub fn get_matches(cli: &Config, package_info: &PackageInfo) -> crate::Result<Ma
         Some(&about),
         cli,
     );
-    match app.try_get_matches() {
+
+    let matches = if let Some(args) = args {
+        app.try_get_matches_from(args)
+    } else {
+        app.try_get_matches()
+    };
+
+    match matches {
         Ok(matches) => Ok(get_matches_internal(cli, &matches)),
         Err(e) => match e.kind() {
             ErrorKind::DisplayHelp => {